One of the important causes of the failure of renal transplantation is cytomegaloviral infection, If such infection, in either active or carrier state in donor kidney or recipient himself, be treated or suppressed, the success rate
of
renal transplantation would increase significantly. There are several antiviral agents available but none of them is specifically effective. the LAK cell, which can be produced by adding recombinant interleukin-2 while culturing
peripheral
mononuclear cells, is known to have antiviral activity to certain virus but not yet to cytomegalovirus. For that reason, the author carried out this experiment to find out whether the LAK cell has such killing or suppressing effect to the
cytomegalovirus using the cytomegaloviral infected MRC-5 cell line, and obtained the following results.
1. Nylon wool nonadherent cells(NWNC) of peripheral mononuclear cells were treated by 1000U/ml of recombinant interleukin-2 and incubated for 5 days under 37。C, 5% CO2. T cells among NWNC decreased from 64. 3% to 55.71%, B cells
slightly
increased from 2.37% to 2.72%. CD 56 positive cells (LAK cell) were increased from 19.06% to 73.70%.
2. To select the best method for evaluating the killing or cytolytic effect of effector cell, manual trypan blue method and automated propidium iodide flow cytometric method were evaluated. And found that the dead cell % of MRC-5
cell
by
trypan blue method and by propidium iodide using flow cytometer were comparable and statistically well correlated(r>0.8).
3. In case the full blown cytopathic effect was shown in MRC-5 cell line, where treated with LAK cell, comparing to the dead cell % of the control MRC-5 cell group, the CMV infected MRC-5 cell group were observed to be higher in target to
effector
ratios being 1 : 1 through 1 : 40 except 1 : 10. and the average dead cell % of all groups between the MRC-5 cell lines and the CMV infected MRC-5 cell line were statistically significantly different(p<0.05).
4. In case the cytopathic effect were initially shown MRC-5 cell line, where treated with LAK cell, comparing to the dead cell % of the control MRC-5 cell group, and CMV infected MRC-5 cell group were observed to appear higher in target
to
effector ration being 1 : 2.5 to 1: 10, but the average dead cell % of all groups between the MRC-5 cell line the CMV infected MRC-5 cell line were not statistically significantly different(p>0.05).
In conclusion, in case the ratios between the CMV-infected cell(target cell) and the LAK cell(effector cell) were from 1 : 2.5 to 1 :10, the LAK cell appears to have the killing effect to the CMV-infected cell regardless the severity of
CMV
infection.
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